The microplate reader also known as “Photometric micro-plate reader or ELISA reader” is a specialized spectrophotometer designed to read results of the ELISA test, a technique used to determine the presence of antibodies or specific antigens in samples. The technique is based on the detection of an antigen or antibodies captured on a solid surface using direct or secondary, labelled antibodies, producing a reaction whose product can be read by the spectrophotometer. The word ELISA is the acronym for “Enzyme-Linked Immunosorbent Assay”. This chapter covers the use of microplate readers for ELISA testing. For additional information on the instrument principles of operation and maintenance, consult Chapter 11 discussing the spectrophotometer.
Microplate Reader, UMDNS: 12536